Multi-components Quantitation by One Marker for Simultaneous Content Determination of Four Components in Psoralea corylifolia

Objective:To explore feasibility of multi-components quantitation by one marker(QAMS) used in quality evaluation of Psoralea corylifolia.Method:P.corylifolia was chosen as the research object,with psoralen as index,and the relative correction factors(RCF) of psoralen-isopsoralen,psoralen-corylifolin(bavachin) and psoralen-isobavachalcone were determined by HPLC.Difference of QAMS and external standard method was investigated,effects of different instrument and column on RCF was investigated.Result:RCF of psoralen-isopsoralen,psoralen-corylifolin(bavachin) and psoralen-isobavachalcone were 0.863 0(1.17%),0.299 6(0.86%),0.201 3(0.37%),respectively.No significant difference were found in quantitative results of four components in P.coryfolia determined by external standard method and QAMS.Conclusion:It is feasible and suitable to evaluate the quality of P.coryfolia by QAMS.