The optimal conditions of polyphenols extraction from kiwifruit were studied in this paper. The effects of five factors including organic solvent and its concentration, extraction time, temperature, extraction period and the ratio of raw materials and solution on extraction rate of polyphenols were investigated. The best technical conditions for polyphenols extraction was obtained through orthogonal experiments based on single factor as follows: extracting with 30 % acetone solution, ratio of raw materials and solution was 1∶9, temperature at 35 ℃ extracting 30 min.