Isolation and PCR identification of Salmonella and Shiga Toxin-producing Escherichia coli from Tibetan Sheep

The objective of this study was to isolate Salmonella and Shiga toxin-producing Escherichia coli from healthy fecal swabs of Tibetan sheep.Total 19 fecal swabs were collected to isolate Salmonella and Escherichia coli with selective media.One Salmonella strain and 4 Shiga toxin-producing Escherichia coli were identified with PCR primers designed according to Salmonella invasion protein A gene(invA),Escherichia coli Shiga-like toxin gene 1and 2,respectively.The invA gene homology of the isolated Salmonella was 99.8% with 5 selected serotypes of Salmonella recorded in NCBI database,and the stx1 and stx2 homology of the 4 Shiga toxin-producing Escherichia coli isolates recored in GenBank were above 95%.This study established the bases for the epidemiology of Salmonella and Shiga toxin-producing Escherichia coli originated from Tibetan sheep.