导管内乳头状粘液性肿瘤
恶性肿瘤
发育不良
激光捕获显微切割
医学
病理
组织微阵列
显微解剖
癌症研究
基因
免疫组织化学
内科学
基因表达
生物
胰腺
生物化学
作者
Robert P. Jury,Bryan J. Thibodeau,Laura E. Fortier,Timothy J. Geddes,Samreen Ahmed,Barbara L. Pruetz,Maryam A. Farinola,George S. Wilson
出处
期刊:Pancreas
[Ovid Technologies (Wolters Kluwer)]
日期:2012-05-01
卷期号:41 (4): 611-618
被引量:13
标识
DOI:10.1097/mpa.0b013e31823d7b36
摘要
The diagnosis of high-grade intraductal papillary mucinous neoplasm (IPMN) is difficult to distinguish from low-grade IPMN. The aim of this study was to identify potential markers for the discrimination of high-grade and invasive (HgInv) IPMN from low- and moderate-grade dysplasia IPMN.Laser capture microdissection was used to isolate distinct foci of low-grade, moderate-grade, high-grade, and invasive IPMN from paraffin-embedded archival tissue from 14 patients who underwent resection for IPMN. Most samples included multiple grades in the same specimen. Affymetrix Human Exon microarrays were used to compare low- and moderate-grade dysplasia IPMN with HgInv IPMN.Sixty-two genes were identified as showing significant changes in expression (P ≤ 0.05 and a 2-fold cutoff), including up-regulation of 41 in HgInv IPMN. Changes in gene expression are associated with biological processes related to malignant behavior including cell motion, cell proliferation, response to hypoxia, and epithelial-to-mesenchymal transition. In addition, altered signaling in several transforming growth factor β-related pathways was exhibited in the progression of IPMN to malignancy.This study identifies a set of genes associated with the progression of IPMN to malignancy. These genes are potential markers that could be used to identify IPMN requiring surgical resection.
科研通智能强力驱动
Strongly Powered by AbleSci AI