Cyclophosphamide were taken as comparison, S180tumor transplanting model was established to assay the inhibitory effect of polysaccharide from Korea and Japan Phellinus igniarius fruit-body. Gas chromatography(GC) was adopted to analyse the composition of monosaccharide. The result shows that: Both of polysaccharides showed potent anti-tumor effect, the rates of anti-tumor were 52.36%, 72.64%. It showed Phellinus igniarius fruit-body polysacchari-de of Korea was consisted of five monosaccharides, which are rhamnose, arabinose, glucose,mann-ose and galactose, the Japan was consisted of six monosaccarides, which are rhamnose,arabinose, xylose, glucose,mannose and galactose.