Effects of Bioactive Plant Components on Cell Viability and Comparison of Cell Viable Assays

Resveratrol and chlorogenic acid as phytochemicals were used to treat CHO cells.Cell viability was measured by using MTS,WST-1,CCK-8,XTT and MTT assays.Comparison of MTT assay with four others was carried out to screen the optimal cell viability array.Along with this,the cytotoxicity of resveratrol and cholorogenic acid was evaluated by IC50.The CCK-8 assay provided the most similar IC50to the MTT assay with good stability and consequently was the best choice.In contrast,the WST-1 assay showed worst repeatability and smaller results.The XTT assay was the least stable and less repeatable and the optimal reaction time and detection wavelength was the most difficult to determine.The IC50 values of resveratrol were 116.86,124.68,64.90,162.86 μmol/L and 86.30 μmol/L respectively,and the IC50 values of chlorogenic acid were 2808.60,1119.96,1029.72,1325.43 μmol/L and 2852.63 μmol/L respectively as determined by the CCK-8,MTS,WST-1,XTT and MTT assays.These data indicated that higher IC50value could be obtained for chlorogenic acid by each assay compared with resveratrol.In light the above findings,we conclude that the CCK-8 assay provides the best approach to determining the effects of bioactive plant components on cell viability and that resveratrol has higher cytotoxicity to CHO cells than chlorogenic acid.