Determination of melamine in milk powder by chemiluminescence method

Melamine is able to significantly enhance the chemiluminescence reaction of luminal and K3Fe(CN)6 in alkaline medium.This makes it feasible to establish a new flow injection chemiluminescence method for determining melamine in milk powder.The optimal condition for the determination was examined,and as-established method was adopted to determine melamine in commercial milk powder.It was found that under the optimal experimental condition,melamine concentration in the range of 1.0×10-6-4.0×10-5 g · L-1 was linearly proportional to enhancing chemiluminescence signals.The detection limit for melamine was determined to be 2.6×10-7 g · L-1,and the relative standard deviation for 11 consecutive measurements of melamine with a concentration of 4.0×10-6 g · L-1 was 3.42%.In the meantime,as-established method was successfully applied to determine melamine in commercial milk powder.