The specificity of multi-column liquid chromatography of neutral urinary steroids as evaluated by comparison with parallel gas chromatography and mass spectrometry.

The use of gas chromatography/mass spectrometry is advocated as the best currently available methodology for the validation of a routine assay for steroid analysis. This is documented by comparative studies by gas chromatography/mass spectrometry and spectrophotometry of peak material obtained in routine assays by multi-column liquid chromatographic techniques.