Objective:To establish a method for content determination of polysaccharides in the root of Panax ginseng.Method:The conversion coefficient of ginseng polysaccharide to glucose was obtained by refined polysaccharides,and then the content of crude polysaccharides in the root of Panax ginseng was determined by the method of phenol-sulferic acid.Result:the color of the treated solution showed good stabilization within 2h and the average value of the recovery for polysaccharide measurement was 99.8% with 2.57% of RSD.The contents of polysaccharide in the root of Panax ginseng was 28.56% with 1.81% of RSD.Conclusion :The method was simple,rapid and accurate.