Objective To realize the correlation between cytomegalovirus infection and patients with neonatal jaundice.Methods The serum IgM and IgG antibody against cytomegalovirus of patients with neonatal jaundice was detected by enzyme-linked immunosorbent assay(ELISA).Results The positive rates of anti-CMV antibody(included 2 cases IgM and 14 cases of IgG positive serum) in patients was 22.22%(16/72).Conclusions There was correlation between cytomegalovirus infection and neonatal jaundice,CMV infection probably was one of the pathogenic factor of patients with neonatal jaundice.