顺铂
鼻咽癌
细胞培养
流式细胞术
卡铂
细胞
MTT法
倍增时间
细胞生长
奥沙利铂
癌症研究
化学
生物
免疫学
医学
化疗
癌症
内科学
生物化学
放射治疗
结直肠癌
遗传学
作者
Wen-Hsin Lin,Derui Li,Yingcheng Lin,Chenxing Du,Jiongyu Chen,Chao‐Qun Hong
摘要
To establish a CDDP-resistant cell line from human nasopharyngeal carcinoma and evaluate its biological characteristics.By continuously exposing and gradually increasing dose of cisplatin (CDDP), a resistant nasopharyngeal carcinoma cell line (HNE1/CDDP) was established. Drug sensitivity of this cell line was detected by MTT assay; the alterations of its biological characteristics were determined using light microscopy, cell counting and flow cytometry (FCM); its ability of adhesion, migration and invasion were also evaluated.HNE1/CDDP cell line was developed after 10 months with stable resistance to cisplatin with the resistance index was 5.83. HNE1/CDDP cell exhibited cross-resistance to many other chemotherapeutic agents (carboplatin, oxaliplatin and etoposide, etc). The morphology of HNE1/CDDP changed; doubling time prolonged; and the cell number of S-phase and G2/M-phase decreased while of G0/G1 phase increased compared with parental cells. The ability of adhesion, migration and invasion had no difference between the parental and the resistant cells.HNE1/CDDP cell line shows the typical and stable resistant phenotype and can be used as a research model.
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