A Novel Label-Free Method for Efficient Detection of Bisphosphonate Drug Dissolution Rates Using Surface-Enhanced Raman Spectroscopy

We developed a novel method utilizing surface-enhanced Raman spectroscopy (SERS) with calcium ion-modified silver nanoparticles as the enhancing substrate. This approach enables label-free detection of the dissolution rate of bisphosphonates. This technique is fast, sensitive, and highly reproducible, significantly advancing drug quality control. Experimental results demonstrated detection limits for etidronate, alendronate, and risedronate as low as 1 ng/mL. Notably, this method accurately identifies characteristic drug peaks without interference from excipients, allowing for precise quantitative detection. In summary, our SERS-based method offers a simple, efficient, and reliable approach for the qualitative and quantitative quality control of bisphosphonate drugs.