细胞
低温保存
细胞生物学
单细胞分析
化学
生物
胚胎
生物化学
作者
Valeria Garcia‐Flores,Derek Miller,José Galaz,Nardhy Gomez‐Lopez
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 143-154
标识
DOI:10.1007/978-1-0716-3746-3_13
摘要
The dissociation of whole tissue into single-cell suspensions is a critical step for techniques focused on profiling of individual cells. Here, we describe a protocol for the preparation of high-quality single-cell suspensions from human placental tissues: the basal plate (BP), placental villi (PV), and chorioamniotic membranes (CAM). This protocol also provides guidance for the cryopreservation and recovery of single-cell suspensions for later use. The methods described here have been demonstrated to be suitable for downstream single-cell applications, such as single-cell RNA-sequencing, that require viable, high-quality cell suspensions.
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