拉伤
氮气
硝酸盐
酶
化学
铵
细菌
亚硝酸盐
生物化学
微生物学
生物
遗传学
有机化学
解剖
作者
Xin Kang,Xiaoxiang Zhao,Xinshan Song,Danhe Wang,Guitao Shi,Xiaohui Duan,Xiaohua Chen,Guoqing Shen
标识
DOI:10.1016/j.procbio.2023.05.030
摘要
To boost the poor nitrogen removal capacity of microorganisms under alkaline and low-temperature conditions, a new strain Priestia aryabhattai KX-3 able to heterotrophically nitrify and aerobically denitrify (HN-AD) was isolated from the Larsemann Hills in East Antarctica. The unique HN-AD function described herein has never been reported previously. At 10 ℃ and pH 10.0, strain KX-3 can remove 96.0% of nitrate–N (40 mg/L), 92.5% of ammonium–N (40 mg/L), and 56.7% of nitrite–N (40 mg/L). In addition, functional genes (amoA, hao, napA, nirS, and nosZ) and three key enzymes related to nitrogen reduction (NIR, NR and HAO) were detected, confirming the HN-AD abilities of strain KX-3. Finally, transcriptomics data confirmed the up-regulation of genes associated with the GDH2 pathway, such as gln A and gltBD. In addition, the genes for the cold-resistant ABC transporters and Na+/H+ antiporters (MnhACDEFG, NhaCD, and TrkAH) were expressed highly. Strain KX-3 has a strong potential for nitrogen removal under low temperature and alkaline conditions. Moreover, it also provides reference value for research on microbial tolerance to extreme conditions in East Antarctica.
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