Hyaluronan-based Hydrogels for 3D Modelling of Tumour Tissues

Although routine 2D cell culture techniques have advanced basic cancer research owing to their simplicity, cost-effectiveness, and reproducibility, they have limitations that necessitate the development of advanced 3D tumour models that better recapitulate the tumour microenvironment. Various biomaterials have been used to establish these 3D models, enabling the study of cancer cell behaviour within different matrices. Hyaluronic acid (HA), a key component of the extracellular matrix in tumour tissues, has been widely studied and employed in the development of multiple cancer models. This review first examines the role of HA in tumours, including its function as an extracellular matrix (ECM) component and regulator of signalling pathways that affect tumour progression. It then explores HA-based models for various cancers, focusing on HA as a central component of the 3D matrix and its mobilization within the matrix for targeted studies of cell behaviour and drug testing. The tumour models discussed included those for breast cancer, glioblastoma, fibrosarcoma, gastric cancer, hepatocellular carcinoma, and melanoma. The review concludes with a discussion of future prospects for developing more robust and high-throughput HA-based models to more accurately mimic the tumour microenvironment and improve drug testing.