A new secondary metabolite from the marine-derived fungus Phomopsis lithocarpus FS508

AbstractBased on the One Strain-Many Compounds (OSMAC) strategy, the secondary metabolites of Phomopsis lithocarpus FS508 were investigated. As a result, a new secondary metabolite, 4-methoxy-3-[4-(acetyloxy)-3-methyl-2-butenyl]benzoic acid (1) as well as eleven known compounds were isolated from the fermentation product of the strain FS508. Their structures were determined by NMR, IR, UV, and MS spectroscopic data analyses. All the isolated compounds were evaluated for cytotoxic and anti-inflammatory activities. Among them, compounds 3 and 9 displayed potent cytotoxicity against HepG-2 cell line, and compounds 2, 3 and 12 showed significant anti-inflammatory activities.Keywords: Marine fungusPhomopsis lithocarpusOSMACcytotoxicityanti-inflammatory AcknowledgementsWe sincerely thank Ms. Ai-Jun Sun, Yun Zhang and Xuan Ma of the South China Sea Institute of Oceanology, and the Chinese Academy of Sciences, for the measurement of HRESIMS, UV and IR.Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was financially supported by Guangdong Special Support Program (2019TQ05Y375), Guangdong Provincial Special Fund for Marine Economic Development Project ([2021]054), and Guangdong Provincial Project for Science and Technology (No. 2021A1515011549).