铁载体
苗木
假单胞菌
细菌
生物
开枪
磷酸盐
溶磷菌
生物肥料
培养皿
植物
吲哚-3-乙酸
园艺
生长素
微生物学
根际
根际细菌
生物化学
基因
遗传学
作者
Roberto Lanna‐Filho,Bruna Canabarro Pozzebon,Andréia Mara Rotta de Oliveira
标识
DOI:10.1590/1808-1657000162021
摘要
Endophytic bacteria Bacillus safensis RS95 and Pseudomonas hibiscicola RS121 were evaluated for their ability to promote the growth of rice seedlings and produce indole-acetic acid (IAA) and siderophores and to solubilize phosphates. ‘Guri’ rice seeds were immersed in bacterial endophyte cell suspensions (separated and two-strain mixed), as well as in Escherichia coli DH5α, phosphate-buffered saline (PBS) and water treatments (negative controls). Seeds were sown on agar-water in Petri plates placed vertically at an angle of 65°. The ability of plant growth-promoting endophytic bacteria (PGPEB) to produce IAA and siderophores was determined by Salkowski colorimetric and chrome azurol S (CAS) assays, respectively. Mineral phosphate solubilization activity was calculated by inoculating the endophytes onto medium containing insoluble phosphate. PGPEB showed a positive effect on the growth of rice seedlings, causing a mean growth of shoots and primary-roots of 60 and 67%, respectively. Bacterial strains also showed positive traits for IAA and siderophore production, as well as phosphate-solubilization activity.
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