细胞凋亡
利多卡因
标记法
平衡盐溶液
体内
台盼蓝
眼科
医学
男科
角膜
曲安奈德
内皮干细胞
细胞
化学
体外
生物
外科
生物化学
有机化学
生物技术
作者
Sezin Akça Bayar,Zeynep Kayaarası Öztürker,Yonca A. Akova,Banu Bilezikçi,Gülten Karabay
标识
DOI:10.4274/tjo.galenos.2022.64369
摘要
To evaluate the effects of intracameral drugs and dyes on rat corneal endothelial apoptosis and cell morphology.The right eyes of 72 rats were injected intracamerally with 1% lidocaine, 0.01% adrenaline, triamcinolone acetonide (TA) 4 mg/mL, 1% trypan blue (TB), 0.5% indocyanine green (ICG), and fortified balanced salt solution as control. Corneal samples were taken 1 day and 1 week post-injection. Corneal endothelial apoptosis was assessed by the TUNEL technique, and the ratio of apoptotic cells in each group was compared with the control. Corneal endothelial cell morphology was evaluated in each specimen by transmission electron microscopy.The mean apoptotic endothelial cell ratio was significantly higher at 1 day and 1 week after intracameral adrenaline injection when compared to controls (p=0.03 and 0.021, respectively). TB caused a significantly higher apoptotic cell ratio when compared to controls at 1 week after injection (p=0.043). Lidocaine caused a higher apoptotic cell ratio compared to TA and ICG at 1 week, although not statistically significant (p=0.058, 0.09, 0.69, respectively). In all experimental specimens, transmission electron microscopy showed morphological changes associated with apoptosis.This study showed that intracameral adrenaline, TB, and lidocaine injections may have toxic effects on corneal tissue, as indicated by ultrastructural and histopathological alterations. Therefore, these agents should be used with caution in intraocular surgery.
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