大肠杆菌
枯草芽孢杆菌
细菌
脱氧核酶
核酸
检出限
肺炎克雷伯菌
化学
DNA
微生物学
生物传感器
计算生物学
生物
组合化学
纳米技术
色谱法
生物化学
材料科学
遗传学
基因
作者
Sara M. Imani,Enas Osman,Fatemeh Bakhshandeh,Shuwen Qian,Sadman Sakib,Michael J. MacDonald,Mark Gaskin,Igor Zhitomirsky,Deborah Yamamura,Yingfu Li,Tohid F. Didar,Leyla Soleymani
标识
DOI:10.1002/advs.202207223
摘要
Abstract There is a need for point‐of‐care bacterial sensing and identification technologies that are rapid and simple to operate. Technologies that do not rely on growth cultures, nucleic acid amplification, step‐wise reagent addition, and complex sample processing are the key for meeting this need. Herein, multiple materials technologies are integrated for overcoming the obstacles in creating rapid and one‐pot bacterial sensing platforms. Liquid‐infused nanoelectrodes are developed for reducing nonspecific binding on the transducer surface; bacterium‐specific RNA‐cleaving DNAzymes are used for bacterial identification; and redox DNA barcodes embedded into DNAzymes are used for binding‐induced electrochemical signal transduction. The resultant single‐step and one‐pot assay demonstrates a limit‐of‐detection of 10 2 CFU mL −1 , with high specificity in identifying Escherichia coli amongst other Gram positive and negative bacteria including Klebsiella pneumoniae , Staphylococcus aureus , and Bacillus subtilis . Additionally, this assay is evaluated for analyzing 31 clinically obtained urine samples, demonstrating a clinical sensitivity of 100% and specify of 100%. When challenging this assay with nine clinical blood cultures, E. coli ‐positive and E. coli ‐negative samples can be distinguished with a probability of p < 0.001.
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