佩多:嘘
材料科学
石墨烯
原位
电化学
纳米技术
晶体管
形态学(生物学)
电极
化学工程
化学
生物
电压
有机化学
物理
量子力学
工程类
遗传学
图层(电子)
物理化学
作者
Qingyuan Song,Hongni Liu,Weiyi Wang,Chen Chen,Yiping Cao,Bolei Chen,Bo Cai,Rongxiang He
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2024-01-01
卷期号:16 (7): 3631-3640
摘要
Circulating tumor cells in human peripheral blood play an important role in cancer metastasis. In addition to the size-based and antibody-based capture and separation of cancer cells, their electrical characterization is important for rare cell detection, which can prove fatal in point-of-care testing. Herein, an organic electrochemical transistor (OECT) biosensor made of solution-gated carboxyl graphene mixed with PEDOT:PSS for the detection of cancer cells in situ is reported. Carboxyl graphene was used in this work to modulate cancer cell morphology, which differs significantly from normal blood cells, to achieve rare cancer cell detection. When the concentration of carboxyl graphene mixed in PEDOT:PSS was increased from 0 to 5 mg mL-1, the cancer cell surface area increased from 218 μm2 to 530 μm2, respectively. A change in cell morphology was also detected by the OECT. Negative charges in the cancer cells induced a positive shift in gate voltage, which was approximately 40 mV for spherical-shaped cells. When the cell surface area increased, transfer curves of transistor revealed a negative shift in gate voltage. Therefore, the sensor can be used for in situ detection of cancer cell morphology during the cell capture process, which can be used to identify whether the captured cells are deformable.
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