脐静脉
血管生成
体内
再生(生物学)
化学
细胞生物学
血管内皮生长因子
信使核糖核酸
血管内皮生长因子A
血管内皮生长因子受体
细胞外小泡
细胞外
癌症研究
单宁酸
细胞凋亡
分子生物学
体外
肿瘤坏死因子α
转录组
新生血管
治疗性血管生成
作者
Yuting Wang,Yulong Zhang,Lin Zhou,Yan Li,Xiaoran Zu,Julei Zhang,Wende Yao,Sihan Yang,Yinan Wang,Dongsheng Chen,Yan Han,Linsheng Zhan,Yan Han
出处
期刊:Small
[Wiley]
日期:2025-10-31
卷期号:21 (50): e08210-e08210
被引量:1
标识
DOI:10.1002/smll.202508210
摘要
Prefabricated flaps often suffer from distal necrosis due to ischemia, with no clinically effective prevention or treatment currently available. Although extracellular vesicles (EVs) derived from adipose-derived stem cells (ADSCs) have shown pro-angiogenic potential, their therapeutic efficacy alone remains limited. In this study, VEGF mRNA is successfully encapsulated within ADSCs-derived EVs, which subsequently exhibit pro-angiogenic effects on cultured human umbilical vein endothelial cells (HUVECs). To enable sustained release, a biocompatible tannic acid-based hydrogel (TA-Gel) is developed with tunable mechanical properties and antimicrobial activity. This hydrogel significantly enhances both flap viability and vascular regeneration in vivo when combined with VEGF-EVs. Transcriptome sequencing reveals that VEGF-EVs@TA-Gel upregulates differentially expressed genes (DEGs) involved in VEGF-related pro-angiogenesis, collagen response, and anti-oxidative stress pathways. Moreover, 16S rRNA sequencing confirms that VEGF-EVs@TA-Gel inhibits the growth of common pathogenic bacteria, including Escherichia coli and Pseudomona. Collectively, these findings indicate that VEGF-EVs@TA-Gel promotes the survival and quality of prefabricated flaps through the sustainable release of VEGF mRNA-loaded EVs.
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