根(腹足类)
PI3K/AKT/mTOR通路
中医药
药理学
传统医学
蛋白激酶B
氧化应激
系统药理学
机制(生物学)
小檗碱
黄芪
化学
信号转导
医学
生物
细胞生物学
生物化学
药品
病理
植物
替代医学
哲学
认识论
作者
Pin Gong,Danni Wang,Dandan Cui,Qian Yang,Peipei Wang,Wenjuan Yang,Fuxin Chen
出处
期刊:Phytomedicine
[Elsevier]
日期:2021-04-01
卷期号:84: 153509-153509
被引量:34
标识
DOI:10.1016/j.phymed.2021.153509
摘要
Radix Astragali (RA) consists of the dried root of Astragalus membranaceus Bunge and is one of the most frequently used dietetic Chinese herbs to treat inflammation and neurodegenerative disease among other conditions. Radix Astragali preparata (RAP) is a medicinal form of RA. RA and RAP have been used as anti-aging agent, however, the mechanisms underlying their effects are still unclear. Considering the wide application of RA and RAP in clinical practice, it is necessary to identify the better product between the two and elucidate the molecular mechanism responsible for their anti-aging effects. In this study, network pharmacology integrated with molecular biology techniques were employed to explore the possible mechanism of RA and RAP against aging. Aging animal models were constructed by exposure to D-galactose (D-gal), and the anti-aging effect of RA and RAP were determined based on behavior tests and histomorphological observation. Network pharmacology was performed to construct the “compound-target-pathway” network. Gene and protein expression of possible targets were validated and analyzed using qRT-PCR and Western blotting. Treatment by RA and RAP could alleviate the symptoms of aging such as a decrease in body weight and organ indices, behavioral impairment, increased oxidative stress, weaken histopathological evaluation. The effect of RAP was more pronounced than that of RA in preventing aging process in a mouse model. The anti-aging effect of RA and RAP is associated with the balance of oxidative stress and activation of PI3K/Akt signaling pathway. Using an integrated strategy of network pharmacology and molecular biology we attempted to elucidate the mechanisms of action of RA and RAP.
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