Abscisic acid signaling negatively regulates nitrate uptake via phosphorylation of NRT1.1 by SnRK2s in Arabidopsis

拟南芥 脱落酸 硝酸盐 拟南芥 磷酸化 化学 细胞生物学 野生型 生物化学 突变体 激酶 生物 基因 生态学
作者
Hang Su,Tian Wang,Chuanfeng Ju,Jinping Deng,Tian‐Qi Zhang,Mengjiao Li,Hui Tian,Cun Wang
出处
期刊:Journal of Integrative Plant Biology [Wiley]
卷期号:63 (3): 597-610 被引量:56
标识
DOI:10.1111/jipb.13057
摘要

Abstract Nitrogen (N) is a limiting nutrient for plant growth and productivity. The phytohormone abscisic acid (ABA) has been suggested to play a vital role in nitrate uptake in fluctuating N environments. However, the molecular mechanisms underlying the involvement of ABA in N deficiency responses are largely unknown. In this study, we demonstrated that ABA signaling components, particularly the three subclass III SUCROSE NON‐FERMENTING1 (SNF1)‐RELATED PROTEIN KINASE 2S (SnRK2) proteins, function in root foraging and uptake of nitrate under N deficiency in Arabidopsis thaliana . The snrk2.2snrk2.3snrk2.6 triple mutant grew a longer primary root and had a higher rate of nitrate influx and accumulation compared with wild‐type plants under nitrate deficiency. Strikingly, SnRK2.2/2.3/2.6 proteins interacted with and phosphorylated the nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) in vitro and in vivo . The phosphorylation of NRT1.1 by SnRK2s resulted in a significant decrease of nitrate uptake and impairment of root growth. Moreover, we identified NRT1.1 Ser585 as a previously unknown functional site: the phosphomimetic NRT1.1 S585D was impaired in both low‐ and high‐affinity transport activities. Taken together, our findings provide new insight into how plants fine‐tune growth via ABA signaling under N deficiency.
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