内吞作用
内化
归巢(生物学)
间充质干细胞
干细胞
化学
内吞循环
细胞生物学
超顺磁性
PLGA公司
纳米颗粒
内体
生物物理学
纳米技术
网格蛋白
胞饮病
细胞
材料科学
生物化学
生物
量子力学
磁场
物理
磁化
生态学
作者
Su Hoon Lee,Dong Jun Park,Wan Su Yun,Jeong-Eun Park,Jin Woo Choi,Jaehong Key,Young Joon Seo
标识
DOI:10.1016/j.jconrel.2020.07.032
摘要
The technology of directing nanoparticles to specific locations in the body continues to be an area of great interest in a myriad of research fields. In the present study, we have developed nanoparticles and a method that allows the nanoparticles to move to specific sites by simultaneously utilizing the homing ability and magnetism of stem cells. Polymeric clustered SPIO (PCS) nanoparticles are composed of a superparamagnetic iron oxide nanoparticle (SPION) cluster core coated with poly lactic-co-glycolic acid (PLGA) and labeled with the fluorescent dye Cy5.5 for tracking. PCS is designed to be internalized by stem cells via endocytosis and then moved to the desired subcellular location through magnetism. Here, we investigated the interactions between SPIONs and mesenchymal stem cells (MSCs), including their absorption mechanism and subcellular localization. Exposure to the nanoparticles at 40 μg/mL for over 96 h did not affect cell survival or differentiation. We used a variety of endocytosis inhibitors and identified the potential cellular internalization pathway of SPIONs to be clathrin-mediated endocytosis. Antibodies to organelles were used to accumulate lysosomes through early and late endosomes. PCS at 40 μg/mL was internalized and stored without significant deleterious effects on stem cells, indicating that MSCs can act as an effective nanoparticle carrier. These findings also demonstrate the successful localization of the novel particles using magnetic attraction.
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