超声
壳聚糖
化学
分散性
Zeta电位
纳米颗粒
核化学
甲氨蝶呤
粒径
植酸
离子强度
色谱法
生物化学
纳米技术
水溶液
有机化学
材料科学
物理化学
生物
免疫学
作者
Yhor Ciro,John Rojas,Ana Laura Di Virgilio,Maria J. Alhajj,Gustavo A. Carabali,Constaín H. Salamanca
标识
DOI:10.1016/j.carbpol.2020.116436
摘要
Methotrexate-loaded phytic acid-chitosan nanoparticles were synthesized by ionic gelation assisted by high-intensity sonication. The nanoparticles were characterized by particle size, polydispersity index, zeta potential (ZP) and encapsulation efficiency. Their physical stability was evaluated at 4 °C and 40 °C, whereas the in-vitro methotrexate release was assessed at pH 7.4. The data were heuristically fit to first-order, Higuchi, Peppas-Sahlin and Korsmeyer-Peppas models of release kinetics. Anticancer activity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay on HT-29 human colon adenocarcinoma cells. Physicochemical analysis showed that the nanoparticles presented positive ZP values, sizes less than <300 nm and low polydispersity, except for systems formed with low amplitude sonication. The nanoparticles exhibited an adequate physical stability and a capability to modify methotrexate release by a non-Fickian mechanism, resulting in a more pronounced cytotoxic effect than the free drug on HT-29 human colon adenocarcinoma cells.
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