Antibacterial Activity of Silver Nanoparticles Synthesized Using Tyrosine as Capping and Reducing Agent

Synthesis and antibacterial activity of silver nanoparticles (AgNPs) using tyrosine as a capping and reducing agent has been performed.The purpose of this research was to understand the effect of silver nanoparticles on antibacterial activity.AgNPs synthesis was performed by combining silver nitrate solution (AgNO3) as a precursor to amino acid tyrosine and heating it in a boiling water bath until the appearance of a color shift from colorless to yellow.Antibacterial activity test of silver nanoparticles (AgNPs) was performed with the bacteria Bacillus subtilis and Escherichia coli using the well diffusion method in solution.The well diffusion method was carried out by making a hole in the agar base media with a diameter of 6 mm.TEM characterization showed the formation of AgNPs has been successful.It was also known that the AgNPs have a round shape with an average particle size of 13.628 nm.AgNO 3 solution had antibacterial activity greater than silver nanoparticles.Ag + ions were converted into silver nanoparticles to reduce the toxic properties of Ag + so that it was safe for human health.The antibacterial activity in solution has a stronger effect against Bacillus subtilis (gram-positive) than Escherichia coli (gram-negative).