化学
主成分分析
指纹(计算)
色谱法
化学计量学
高效液相色谱法
定量分析(化学)
模式识别(心理学)
层次聚类
人工智能
生物系统
聚类分析
计算机科学
生物
作者
Jun-cheng Liao,Yunshan Wu,Fangfang Xu,Wei-ying Chen,Zuo-Liang Zheng,Xiao-Dong Han,Bo Liu,Shumei Wang,De‐an Guo
标识
DOI:10.1016/j.jpba.2020.113636
摘要
The screening of marker compound is of great significance to the quality control of traditional Chinese medicine (TCM). One approach which combines fingerprint and biological evaluation has developed rapidly. Multi-wavelength fusion fingerprints and antioxidant activity screening are integrated in this study to evaluate the quality of NAODESHENG. Characteristic multiwavelength fusion fingerprints of 14 batches of samples were generated at five different wavelengths and evaluated by quantitative fingerprinting with ultra-performance liquid chromatography (UPLC). In the quantitative fingerprinting method, 21 components in NAODESHENG were qualitatively and quantitatively analyzed by external standard method. The antioxidant activities of these 21 components was determined by pre-column antioxidant activity test. Multivariate statistical methods such as hierarchical clustering analysis and principal component analysis(PCA) was used to reduce the dimensions and variables from a large number of original data to screening marker compound with bioactivity. Based on the above results, it is suggested that 3'-Methoxy Puerarin and 11 other components should be used as the quality marker of NAODESHENG. This study demonstrates the feasibility of multi-wavelength fusion fingerprinting combined with antioxidant activity analysis, which associates quality control with bioactivity, providing a reliable and efficient method for quantitative assessment of TCM quality consistency.
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