生物
16S核糖体RNA
基因组DNA
系统发育树
拉伤
醋酸杆菌
细菌
基因
微生物学
植物
遗传学
解剖
作者
Ju Hye Baek,Kyung Hyun Kim,Ji Young Moon,Soo‐Hwan Yeo,Che Ok Jeon
标识
DOI:10.1099/ijsem.0.004008
摘要
A Gram-stain-negative, obligately aerobic bacterium, designated strain B6 T , was isolated from rice wine vinegar in the Republic of Korea. Cells were non-motile and oval short rods showing catalase-positive and oxidase-negative activities. Growth was observed at 15–45 °C (optimum, 30 °C) and pH 3.5–8.0 (optimum, pH 5.5–6.5). Strain B6 T contained summed feature 8 (comprising C 18 : 1 ω7 c and/or C 18 : 1 ω 6 c ), and C 16 : 0 as major fatty acids and ubiquinone-9 was identified as the sole isoprenoid quinone. The G+C content of the genomic DNA calculated from the whole genome was 53.1 mol%. Strain B6 T was most closely related to Acetobacter pasteurianus LMG 1262 T with very high 16S rRNA gene sequence similarity (100 %) and the strains formed a very close phylogenetic lineage together in phylogenetic trees based on 16S rRNA gene sequences. However, relatedness analyses based on concatenated amino acid sequences of 354 core genes and whole-cell MALDI-TOF profiles showed that strain B6 T may form a distinct phyletic lineage from Acetobacter species. In addition, average nucleotide identity and in silico DNA–DNA hybridization values between strain B6 T and the type strains of Acetobacter species were less than 93.3 and 51.4 %, respectively. The genomic features of strain B6 T were also differentiated from those of closely related Acetobacter type strains. Based on the phenotypic, chemotaxonomic and genomic features, strain B6 T clearly represents a novel species of the genus Acetobacter , for which the name Acetobacter oryzoeni sp. nov. is proposed. The type strain is B6 T (=KACC 21201 T =JCM 33371 T ).
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