斑马鱼
突变体
血管生成
生物
细胞生物学
遗传筛选
基因
血管内皮生长因子A
血管内皮生长因子
分子生物学
生物化学
遗传学
癌症研究
血管内皮生长因子受体
作者
Zigang Cao,Hongcheng Wang,Xiaoyu Mao,Lingfei Luo
标识
DOI:10.1016/j.bbrc.2016.03.051
摘要
The canonical functions of Aminoacyl-tRNA synthetases (AARSs) are indispensable for protein synthesis. However, recent evidence indicates that some AARSs possess additional biological functions (noncanonical functions) related to immune responses and vascular development. Here, we identified a zebrafish cq16 mutant presenting the disorganized vessels with abnormal branching of the established intersegmental vessels (ISVs) as well as aberrant patterning of the brain vascular network after 50 h post fertilization. The cq16 mutant gene is responsible for encoding threonyl-tRNA synthetase (tars) with a missense mutation. The abnormal branching of ISVs was caused by the increased expression of vascular endothelial growth factor A (vegfa) in tarscq16 mutant. Inhibition of Vegf signaling suppresses the abnormal vascular branching observed in tarscq16 mutant. Furthermore, injection of human TARS mRNA potently reduced the vascular aberrant branching in tarscq16 mutant, indicating a conserved function of tars in regulating angiogenesis between zebrafish and human. Therefore, we conclude that noncanonical function of tars regulates vascular development presumably by modulating the expression of vegfa.
科研通智能强力驱动
Strongly Powered by AbleSci AI