In Vivo Monitoring of Microcirculation in Burn Healing Process with Optical Microangiography

Objective: Optical microangiography (OMAG)-based optical coherence tomography is a noninvasive technique capable of imaging functional microvasculature innervating scanned tissue volume. In this study, we utilize OMAG to investigate dynamic changes of microcirculation during the healing process of a burn. Approach: A soft-contact superficial burn injury was induced on a mouse ear with 1 μL 70°C hot water. Microangiograms were generated by using OMAG before and after the burn. Results: Vessel recruitment and remodeling were observed in the healing process. Burn injury reached to the worst extent within the first 24 h and had no expansion thereafter. The interrupted microcirculation in the mouse ear was progressively recovered in the consequent postburn days and completely healed on postburn day 7. Innovation: OMAG provides a novel way for noninvasive visualization and quantification of vasculature changes over time after burn injuries. The high resolution achieved by the imaging system reveals microvascular details down to capillary level. Conclusion: Our results demonstrated that OMAG has great potential to improve the understanding of microcirculatory responses to burns and thus benefit the development of effective therapeutics.