The complete nucleotide sequence of the cloned DNA of hepatitis B virus subtype adr in pADR-1.

The complete nucleotide sequence of the cloned hepatitis B virus DNA subtype adr in pADR-1 was determined by Maxam and Gilbert's method. It is 3215 base pairs in size, which is 27 bp longer than the sequence of the adr pHBr330, as reported by Ono et al. The nucleotide difference between pADR-1 and adr pHBr330 is about 2% while those between pADR-1 and adw as well as ayw are 9.3% and 9.7% respectively. In this paper, the heterogeneity and homogeneity of the S gene, the C gene and the other coding regions in pADR-1 and in the other subtypes are compared and discussed.