发起人
转录因子
DNA结合位点
生物
遗传学
Cis监管模块
基因
基因表达
计算生物学
结合位点
抄写(语言学)
响应元素
基因表达调控
调节顺序
语言学
哲学
作者
Eilon Sharon,Yael Kalma,Ayala Sharp,Tali Raveh-Sadka,Michal Levo,Danny Zeevi,Leeat Keren,Zohar Yakhini,Adina Weinberger,Eran Segal
摘要
Despite extensive research, our understanding of the rules according to which cis-regulatory sequences are converted into gene expression is limited. We devised a method for obtaining parallel, highly accurate gene expression measurements from thousands of designed promoters and applied it to measure the effect of systematic changes in the location, number, orientation, affinity and organization of transcription-factor binding sites and nucleosome-disfavoring sequences. Our analyses reveal a clear relationship between expression and binding-site multiplicity, as well as dependencies of expression on the distance between transcription-factor binding sites and gene starts which are transcription-factor specific, including a striking ∼10-bp periodic relationship between gene expression and binding-site location. We show how this approach can measure transcription-factor sequence specificities and the sensitivity of transcription-factor sites to the surrounding sequence context, and compare the activity of 75 yeast transcription factors. Our method can be used to study both cis and trans effects of genotype on transcriptional, post-transcriptional and translational control.
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