Application of Loop-mediated Isothermal Amplification in Detecting Plant Pathogens

Loop-mediated isothermal amplification(LAMP) was a novel method for nucleic acid amplification with the advantages of high specificity,high sensitivity,simple operation,and fitness for detection of varieties environments,and had been widely used in many fields as a molecular biological detection technology.This paper briefly introduced the principle of LAMP,reviewed its application in detecting plant pathogens,discussed the problems occurring in its application and provided relavent solutions according to these problems,and finally outlooked its application prospect.