Background and Objectives: Preparation and evaluation of nanocomposite films have become prevalent in recent years. Depending on the purpose of using the film, and considering the effects and interaction effects of nanomaterial and other modifiers on the film properties, the amounts of nanomaterials and modifiers should be optimized. The effects of montmorillonite (MMT), glycerol (GL), and their interaction effects on the properties of polyvinyl alcohol-montmorillonite (PVA-MMT) films were evaluated in this research.