适体
卡那霉素
检出限
纳米化学
费斯特共振能量转移
化学
猝灭(荧光)
接受者
分析化学(期刊)
抗生素
荧光光谱法
荧光
光化学
色谱法
分子生物学
生物化学
有机化学
生物
光学
物理
凝聚态物理
作者
Yashan Wang,Tiancong Ma,Shuyue Ma,Yongjun Liu,Yaping Tian,Ruinan Wang,Yanbin Jiang,Dongjun Hou,Jianlong Wang
标识
DOI:10.1007/s00604-016-2011-4
摘要
A rapid and sensitive aptamer-based assay is described for kanamycin, a veterinary antibiotic with neurotoxic side effects. It is based on a novel FRET pair consisting of fluorescent carbon dots and layered MoS2. This donor-acceptor pair (operated at excitation/emission wavelengths of 380/440 nm) shows fluorescence recovery efficiencies reaching 93 %. By taking advantages of aptamer-induced fluorescence quenching and recovery, kanamycin can be quantified in the of 4–25 μM concentration range, with a detection limit of 1.1 μM. The method displays good specificity and was applied to the determination of kanamycin in spiked milk where it gave recoveries ranging from 85 % to 102 %, demonstrating that the method serves as a promising tool for the rapid detection of kanamycin in milk and other animal-derived foodstuff.
科研通智能强力驱动
Strongly Powered by AbleSci AI