Dual-fiber optic bioprobe system for triglyceride detection using surface plasmon resonance sensing and lipase-immobilized magnetic bead hydrolysis

The rapid and accurate detection of triglyceride (TG) plays a valuable role in the prevention and control of dyslipidemia. In this paper, a novel method for TG detection using a dual-fiber optic bioprobe system, which can accurately detect different levels of TG concentration in serum, is proposed. The system employs disposable microprobe-type fiber optic surface plasmon resonance (SPR) biosensors for signal acquisition, providing high stability and portability while avoiding cross-contamination caused by repeated use. The proposed biosensor with a high sensitivity of 1.25 nm/(mg/mL) for TG detection in serum and a tiny diameter of 125 μm, was fabricated using a novel multimode fiber-single-mode fiber-reflector (MSR) structure, which has been scarcely ever reported to the best of our knowledge. In the process of TG detection, lipase-immobilized magnetic beads were introduced to specifically hydrolyze TG, and the relationship between the TG content and the SPR differential signal was obtained from dual-fiber optic bioprobe measurements of the TG sample before and after hydrolysis. The proposed method achieved TG detection in the concentration range of 0-8 mg/mL (including healthy and unhealthy levels of TG concentration in the human body). Additionally, the miniaturized fiber optic biosensors used in this work have the advantages of low sample consumption, high sensitivity, simple operation, label-free measurement, high selectivity, and low cost. This method provides a new pathway for rapid and reliable TG detection and has potential applications in medical research and clinical diagnosis.