吸附
微球
化学工程
材料科学
制作
蛋黄
戊二醛
纳米颗粒
双功能
组氨酸
纳米技术
色谱法
化学
核化学
催化作用
有机化学
酶
病理
替代医学
工程类
医学
食品科学
作者
Yang Wang,Yingying Wei,Qianrui Xu,Shimin Shao,Hong Man,Yingrui Nie,Zhifei Wang,Yong Jiang
出处
期刊:Langmuir
[American Chemical Society]
日期:2021-11-28
卷期号:37 (48): 14167-14176
被引量:1
标识
DOI:10.1021/acs.langmuir.1c02433
摘要
Magnetic materials perform well in the purification of histidine-rich proteins (His-proteins). In this work, a facile fabrication of yolk-shell magnetic Fe3O4@NiSiO3/Ni microspheres for the efficient purification of His-proteins has been reported. Yolk-shell Fe3O4@NiSiO3 microspheres were prepared via hydrothermal reaction. Then Ni nanoparticles (NPs) were loaded on Fe3O4@NiSiO3 microspheres after the adsorption and in situ reduction of nickel acetylacetonate. The yolk-shell Fe3O4@NiSiO3/Ni microspheres had a hierarchical flower-like structure and large cavities. The size of the cavity depended on the reaction time. This indicated that the microspheres had a large specific surface area for loading of more Ni NPs, which was crucial to the high His-protein adsorption capacity of Fe3O4@NiSiO3/Ni microspheres. Fe3O4@NiSiO3/Ni microspheres had a high adsorption capacity for bovine hemoglobin (BHb, 2822 mg/g), which was better than the values of other His-protein adsorbents. Fe3O4@NiSiO3/Ni microspheres still had a high BHb separation efficiency after seven separation cycles, indicating its good reusability and stability. Therefore, the as-prepared bifunctional yolk-shell Fe3O4@NiSiO3/Ni microspheres exhibited great practical application value for His-protein purification.
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