Synthesis of a new environment-sensitive fluorescent probe based on TICT and application for detection of human serum albumin and specific lipid droplets imaging

Environment-sensitive fluorescent probes have always been as forceful tools to understand the pathophysiological processes of relevant diseases. In this work, a new fluorescent probe with typical D-π-A structure was designed and showed high sensitivity to polarity and viscosity changes. DPAR could selectively detect human serum albumin (HSA) with turn-on orange emission in aqueous PBS buffer (pH 7.4), which showed advantages such as rapid response (4 min), high sensitivity (LOD 0.98 μg/mL). Therefore, it was successfully used for achieving HSA levels in urine samples and HSA imaging in HeLa cells. DPAR also exhibited the capability to recognize the cancer cells over the normal cells by lower polarity guided lipid droplets (LDs) imaging (in green emission channel). The detection mechanism for HSA and cancer diagnosis was convinced that DPAR encountered the lower-polarity and higher-viscosity microenvironment, resulting in the confinement of the TICT process and intramolecular rotation. These facts showed that DPAR had good application prospects in environment-related biomedical research and clinical diagnosis.