Smartphone-based photoelectrochemical biosensing system with graphitic carbon nitride/gold nanoparticles modified electrodes for matrix metalloproteinase-2 detection

Photoelectrochemical analysis has been widely used in the field of biosensing due to its high sensitivity and strong anti-interference ability. Herein, a portable and versatile smartphone-based photoelectrochemical biosensing platform was developed for the rapid and on-site biomedical analysis. In the system, light excitation and photocurrent measurements were accomplished by a miniaturized and integrated circuit board. Smartphone with a specifically designed application was utilized to wirelessly control the system via Bluetooth. For photoelectrochemical sensor, graphitic carbon nitride (g-C3N4) and gold nanoparticles loaded on indium tin oxide electrodes were utilized as photoactive materials and signal amplification elements, respectively. The gold nanoparticles were also used to immobilized matrix metalloproteinase-2 (MMP-2) specific cleavage peptide that modified with bovine serum albumin (BSA) on the terminal. In the presence of MMP-2, the peptide was specifically hydrolyzed and cleaved. Thus, parts of the peptide chain and BSA were detached from the electrode resulting in the decrease of steric hindrance and the increase of photoelectrochemical currents. The photocurrents changed linearly with the logarithm of MMP-2 concentrations ranging from 1 pg/mL to 100 ng/mL in both buffer and artificial serum with correlation coefficient of 0.9943 and 0.9698. The limit of detections were as low as 0.48 pg/mL in buffer and 0.55 pg/mL in artifical serum. It indicated that the biosensor has good linearity and high sensitivity, which also verified the effectiveness of the portable instrument. This system provides a pioneering solution for the development of miniaturized and portable photoelectrochemical analysis instruments used for the field monitoring of different analytes.