石墨烯
适体
连接器
生物传感器
场效应晶体管
晶体管
纳米技术
DNA
检出限
材料科学
电解质
化学
分析化学(期刊)
电极
电压
生物化学
分子生物学
色谱法
生物
物理
计算机科学
物理化学
操作系统
量子力学
作者
Rui Campos,Jérôme Borme,J. Rafaela L. Guerreiro,George Machado,M. F. Cerqueira,Dmitri Y. Petrovykh,P. Alpuim
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2019-01-23
卷期号:4 (2): 286-293
被引量:154
标识
DOI:10.1021/acssensors.8b00344
摘要
In this work, we develop a field-effect transistor with a two-dimensional channel made of a single graphene layer to achieve label-free detection of DNA hybridization down to attomolar concentration, while being able to discriminate a single nucleotide polymorphism (SNP). The SNP-level target specificity is achieved by immobilization of probe DNA on the graphene surface through a pyrene-derivative heterobifunctional linker. Biorecognition events result in a positive gate voltage shift of the graphene charge neutrality point. The graphene transistor biosensor displays a sensitivity of 24 mV/dec with a detection limit of 25 aM: the lowest target DNA concentration for which the sensor can discriminate between a perfect-match target sequence and SNP-containing one.
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