Synthesis of a modified peptide fragment analog Val-Tyr (P)-Val-Ala-Ala-OH of cAMP protein kinase regulatory sub unit type II employing Fmoc chemistry

The synthesis of new peptide fragment analog (Val-Tyr(P)-Val-Ala-Ala-OH) incorporating Tyr into the phosphorylation site of the cAMP protein kinase regulatory sub unit type II in place of Scr (Val-Ser(P)-Val-Ala-Ala-OH) has been described. The phosphopentapeptide fragment is prepared by Fmoc chemistry employing the global phosphorylation method. The yield (68%) as well as purity of the final peptide is satisfactory. The peptide is fully characterized by HPLC, NMR and mass spectral data.