脂质体
微泡
小泡
生物分子
化学
外体
生物传感器
检出限
生物物理学
纳米技术
细胞外小泡
膜
色谱法
材料科学
生物化学
细胞生物学
生物
小RNA
基因
作者
Changheon Kim,Kangwon Lee
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2019-08-06
卷期号:20 (9): 3392-3398
被引量:47
标识
DOI:10.1021/acs.biomac.9b00641
摘要
Exosomes are extracellular vesicles (EVs) that have attracted attention because of their important biological roles in intercellular communication and transportation of various biomolecules, including proteins and genetic materials. However, due to difficulties in their selective capture and detection, further application of exosomes remains challenging. To detect EVs, we fabricated a liposomal biosensor based on polydiacetylene (PDA), a conjugate polymer that has been widely used in sensing applications derived from its unique optical properties. To confer selectivity and sensitivity to the sensory material, antibodies targeting CD63, a membrane protein exclusively found in exosomes, were attached to the PDA liposomes and phospholipid molecules were incorporated into the PDA vesicles. Signal analysis derived from PDA liposomes for exosome detection and quantification was performed by observing colorimetric changes triggered by the ligand-receptor interaction of PDA vesicles. Visual, UV-visible, and fluorescence spectroscopic methods were used to obtain signals from the PDA lipid immunosensor, which achieved a detection limit of 3 × 108 vesicles/mL, the minimum concentration that can be used in practical applications. The strategies used in the system have the potential to expand into the field of dealing with exosomes.
科研通智能强力驱动
Strongly Powered by AbleSci AI