A novel turn-on fluorescent probe based on nile red with 2-chloronaphthalene-1,4-dione as receptor for detection of hydrogen sulfide

A novel turn-on fluorescent probe 2-chloro-3-((9-(diethylamino)-5-oxo-5H-benzo[ a ]phenoxazin-2-yl)oxy)naphthalene-1,4-dione ( NR-H 2 S ) that could selectively response to H 2 S, had been designed and synthesized. NR-H 2 S was consist of Nile red as fluorophore and 2-chloronaphthalene-1,4-dione as receptor. Owing to the photo-induced electron transfer (PET) effect, the fluorescence of NR-H 2 S was quenched, while NR-H 2 S exhibited a remarkable “turn-on” fluorescence with the addition of H 2 S. NR-H 2 S displayed good repeatability, strong anti-ion interference ability, high sensitivity and selectivity toward H 2 S, which has been successfully applied to the test strips and detect H 2 S efficiently in food samples, showing its promising prospects. A novel fluorescent probe for the detection of H 2 S based on the Nile Red fluorophore has been designed and synthesized, which exhibited high selectivity, fast response, wide pH adaptability and low detection limit.