淋巴毒素
受体
中国仓鼠卵巢细胞
分子生物学
细胞表面受体
三聚体
生物
淋巴毒素α
BETA(编程语言)
细胞因子
抗体
化学
细胞生物学
生物化学
免疫学
二聚体
有机化学
计算机科学
程序设计语言
作者
Jeffrey L. Browning,Irene Dougas,Apinya Ngam-ek,Paul Bourdon,Barbara Ehrenfels,Konrad Miatkowski,Mohammad Zafari,A M Yampaglia,Pornsri Lawton,Werner Meier
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1995-01-01
卷期号:154 (1): 33-46
被引量:129
标识
DOI:10.4049/jimmunol.154.1.33
摘要
Abstract Lymphotoxin (LT) is a cytokine related to TNF, found in human systems in both secreted and membrane bound forms. The well characterized secreted form is a trimer of a single protein, LT-alpha, whereas the surface form is composed of a complex between two related molecules, LT-alpha and LT-beta. Because there is a distinct receptor for the complex, the membrane form is believed to signal via events different from those elicited by TNF and secreted LT-alpha. By using a battery of anti-LT-alpha and LT-beta mAbs, it is clear that two LT surface forms exist on the surface of PMA-activated II-23 cells, a human T cell hybridoma. Assuming that these surface forms are trimers, a minor form appears early after induction having an apparent stoichiometry of LT-alpha 2/beta 1 and is recognized by one group of anti-LT-alpha mAbs and the p55-TNF receptor. The second and predominant form has an apparent LT-alpha 1/beta 2 composition and is recognized by a second group of pantrophic anti-LT-alpha mAbs and the LT-beta receptor. Neither of the heteromeric forms nor a putative LT-beta homotrimeric form were found to be secreted. The properties of surface LT on the II-23 cell system were similar to those of the surface LT forms on Chinese hamster ovary cells transfected with both LT-alpha and LT-beta genes and a number of lymphoid tumor lines. These experiments point toward the LT-alpha 1/beta 2 complex as the predominant membrane form of LT on the lymphocyte surface, and this complex is the primary ligand for the LT-beta receptor.
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