Dynamic imaging of pharmacokinetics with Sparse Spectral Sampling Stimulated Raman Scattering (S4RS) hyperspectral microscopy

Stimulated Raman Scattering (SRS) is a powerful method for quantifying the uptake, flow, and flux of active pharmaceutical ingredients (APIs). While there are numerous APIs that have unique structures and vibrational spectra, the majority do not have strong or unique vibrational bands. We have developed a sparse spectral sampling SRS imaging system to address these needs that can rapidly and specifically tune into specific Raman shifts from the fingerprint to high wavenumber region in < 5 ms per step. These hyperspectral SRS images can be analyzed using computational analytical approaches to visualize and quantify the dynamics of multiple molecular species simultaneously.