小RNA
下调和上调
RNA结合蛋白
核糖核酸
癌症研究
RNA干扰
污渍
小干扰RNA
免疫系统
和平号-155
NF-κB
生物
信号转导
免疫学
细胞生物学
基因
生物化学
作者
Xingwang Zhao,Rui Dong,Longlong Zhang,Junkai Guo,Ying Shi,Lan Ge,Juan Wang,Zhiqiang Song,Bing Ni,Yi You
标识
DOI:10.1186/s13075-022-02732-x
摘要
Certain circRNAs could be used as biomarkers to determine the risk of development and/or severity of systemic lupus erythematosus, and their new function in the regulation of gene expression has motivated us to investigate their role in SLE METHODS: Experimental methods including qRT-PCR, RNA immunoprecipitation (RIP), pulldown, dual luciferase reporter assay, RNA interference and cell transfection, RNA fluorescence in situ hybridization, western blotting, and mass spectrometry were used to assessed circGARS (hsa_circRNA_0009000) for immune functions and defined mechanisms by which circGARS promotes the progression in SLE.Our results demonstrated that the levels of circGARS was remarkably upregulated in SLE and correlated with clinicopathological features. CircGARS directly combined with microRNA-19a (miR-19a). Functionally, circGARS downregulated the expression of TNFAIP3 (A20, tumor necrosis factor alpha-induced protein 3) to mediate the activation of immune responses that were regulated by the nuclear factor-κB (NF-κB) pathway as a negative feedback mechanism. In addition, miR-19a regulated A20 (TNFAIP3) degradation by downregulating the expression of YTH N6-methyladenosine RNA-binding protein 2 (YTHDF2).The circGARS sponges miR-19a to regulate YTHDF2 expression to promote SLE progression through the A20/NF-κB axis and may act as an independent biomarker to help the treatment of SLE patients.
科研通智能强力驱动
Strongly Powered by AbleSci AI