固定化酶
吸附
酶
化学
共价键
色谱法
磁性纳米粒子
膜
纳米技术
纳米颗粒
材料科学
化学工程
有机化学
生物化学
工程类
作者
Dongmei Li,Juan Chen,Yan‐Ping Shi
标识
DOI:10.1016/j.trac.2018.03.011
摘要
Compared with free enzymes, immobilized enzymes are more robust and resistant to environmental changes. In addition, with enhanced stability, immobilized enzymes can be separated from the reaction mixture and used for repeated cycles. These advantages prompt their applications in various fields. This review outlines the existing methods and easy separated support materials for enzymes immobilization. After a brief introduction on the immobilized enzyme, the immobilization methods of adsorption, entrapment, covalent attachment and cross-linking are discussed. The emphasis is given on the easy separated support materials of magnetic nanoparticles (MNPs), membranes and capillary columns. An outlook on the immobilized enzyme is given at last.
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