高尔基体
内膜系统
细胞生物学
分泌途径
分泌物
细胞器
膜
生物
糖基化
糖蛋白
植物细胞
膜蛋白
内质网
化学
生物化学
基因
作者
Kazusato Oikawa,Takuya Inomata,Yoshitoshi Hirao,Tadashi Yamamoto,Marouane Baslam,Kentaro Kikuchi,Toshiaki Mitsui
出处
期刊:Methods in molecular biology
日期:2017-10-31
卷期号:: 91-105
被引量:3
标识
DOI:10.1007/978-1-4939-7411-5_6
摘要
The Golgi apparatus is an endomembrane system organelle and has roles in glycosylation, sorting, and secretion of proteins in the secretory pathway. It has a central function in living organism and is also essential for plant growth. Proteomic approaches to identify the Golgi membrane proteins have been performed in cell suspension cultures and many Golgi membrane-associated proteins were found, whereas it has well established in rice seedling yet. In this chapter, our recent improving published methods for isolated rice Golgi membranes by floating through a discontinuous sucrose density gradient are provided in detail with proteomic analyses.
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