PLGA公司
材料科学
静电纺丝
间充质干细胞
碱性磷酸酶
细胞外基质
生物医学工程
复合数
纳米纤维
脚手架
组织工程
涂层
复合材料
细胞生物学
纳米技术
化学
生物化学
纳米颗粒
聚合物
生物
医学
酶
作者
Xing Yang,Yuanyuan Li,Wei He,Qianli Huang,Ranran Zhang,Qingling Feng
摘要
Abstract The architecture and composition of bone tissue engineering scaffolds play important roles in modulating the growth of bone tissue. Composite fibers composed of poly(lactic‐co‐glycolic acid) (PLGA) skeleton coated with hydroxyapatite (HA) or hydroxyapatite/collagen (HA/Col) were successfully produced via electrospinning, biomimetic process, and adsorption. The PLGA skeleton fabricated by electrospinning process with a nanofibrous structure (diameter ranging from 200 to 400 nm) showed a morphologic similarity to the extracellular matrix (ECM). SEM, EDX, and XRD analysis confirmed the presence of HA and Col on the composite fibers. Mesenchymal stem cells were used to evaluate the cellular behaviors including cell attachment and spreading, proliferation, and osteogenic differentiation on these fibers (PLGA, PLGA/HA, and PLGA/HA/Col). The results demonstrated that the HA and HA/Col coating improved the interaction between mesenchymal stem cells and the composite fibers reflected by accelerated cell spreading, increased alkaline phosphatase (ALP) activity and enhanced expression of osteogenic‐related genes. The HA/Col coating was more effective in improving this interaction compared with HA coating. The PLGA/HA/Col composite fibers may be promising as a candidate scaffold for bone tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2863–2870, 2018.
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