细胞生物学
丝状体
内质网
肌动蛋白
生物
细胞内
化学
作者
Jitendriya Swain,Peggy Merida,Karla Rubio,David Bracquemond,Aymeric Neyret,Israel Aguilar-Ordoñez,Stefan Günther,Guillermo Barreto,Delphine Muriaux
出处
期刊:iScience
[Elsevier]
日期:2023-07-01
卷期号:: 107384-107384
标识
DOI:10.1016/j.isci.2023.107384
摘要
Our study focused on deciphering the role of F-actin and related regulatory factors during SARS-CoV-2 particle production and transmission in human pulmonary cells. Quantitative high-resolution microscopies revealed that the late phases of SARS-CoV-2 infection induce a strong rearrangement of F-actin nanostructures dependent on the viral M, E, and N structural proteins. Intracellular vesicles containing viral components are labeled with Rab7 and Lamp1 and are surrounded by F-actin ring-shaped structures, suggesting their role in viral trafficking toward the cell membrane for virus release. Furthermore, filopodia-like nanostructures were loaded with viruses, potentially facilitating their egress and transmission between lung cells. Gene expression analysis revealed the involvement of alpha-actinins under the regulation of the protein kinase N (PKN). The use of a PKN inhibitor efficiently reduces virus particle production, restoring endoplasmic reticulum and F-actin cellular shape. Our results highlight an important role of F-actin rearrangements during the productive phases of SARS-CoV-2 particles.
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